RBS
Part:BBa_K784012:Design
Designed by: Ilya Vainberg Slutskin Group: iGEM12_Technion (2012-09-16)
Spacer2 + RBS from theophylline riboswitch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is no source for this part. The RBS is the one used in the theophylline riboswitch.
Source
The spacer region was designed to have minimal secondary structures. In particular, it was designed to have minimal base pairs in the with the RBS, in order to keep it free for interaction with the ribosome. The PacI restriction site in the spacer region was used to self ligate the PCR product, which was a complete plasmid without the aptamer and the additional 5' UTR regions of the theophylline riboswitch.